WebThe high-affinity FXR agonist GW4064 blocks Gly-MCA action in the gut, and intestine-specific Fxr-null mice are unresponsive to the beneficial effects of Gly-MCA. Mechanistically, the metabolic improvements with Gly-MCA depend on reduced biosynthesis of intestinal-derived ceramides, which directly compromise beige fat thermogenic function. WebFigure Legend Snippet: Effect of dioscin and its metabolites on FXR activation by CDCA: ( A ) Activation of FXR by CDCA in the presence of 100 μM ligands (dioscin and its metabolites) to demonstrate the reversibility of binding. ( B ) TR-FRET FXR coactivator recruitment assay to assess whether tigogenin is a functional antagonist to CDCA (100 μM) -mediated …
The Use of the LanthaScreen TR-FRET CAR Coactivator Assay in …
WebCytotoxicity assays were performed to exclude the cytotoxic effects of tested substances on living cells. Based on these tests, the toxicity or effects on viability of many derivatives were confirmed, which we excluded from the subsequent testing. We also performed a TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) cell-free assay WebTR-FRET applications. TR-FRET is used to analyze binding events in biochemical assays. In addition, it is suitable for cell-based assays, since measurements of cell lysates can be performed in the presence of a culture medium (homogeneous detection).. The different biological settings include protein-DNA/RNA interactions, protein-protein (ligand-receptor) … カステラ イラスト フリー素材
Development of coactivator recruitment assays for AR and RXR …
WebThe LanthaScreen TR-FRET FXR (farnesoid X receptor) Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential FXR ligands as agonists of coactivator recruitment. The kit uses a terbium (Tb)-labeled WebShop Invitrogen™ LanthaScreen™ TR-FRET FXR Coactivator Assay Kit, rabbit at Fishersci.nl Invitrogen™ LanthaScreen™ TR-FRET FXR Coactivator Assay Kit, rabbit 800 x 20μL assays Immunochemical Assay Kits Fisher Scientific WebMar 28, 2024 · LanthaScreen™ TR-FRET FXR Coactivator Assay Kit, 800 x 20μL Assays, Green, 384-Well Plate Format, Tb (Terbium) Dye Type, Fluorescent Detection Method, TR-FRET Technique, For Pharma & Biopharma, Target & Lead Identification & Validation: For Use With (Equipment) Microplate reader: Quantity: 800 x 20 μL Assays: Content And … カステライラスト可愛い