WebNov 12, 2024 · The substrate was immobilized as described in the single-molecule degradation assay by the multiturnover reaction. To observe binding, we used Cy3-labeled substrate and Cy5-labeled RNase H instead of Cy3- and Cy5-labeled substrate and nonlabeled RNase H. We used CaCl 2 as a binding cofactor to prevent the degradation … WebDuplex RT-qPCR was performed using primers and probes targeting the indicated viral RNA gene (FAM) and human RNase P (Cy5) on a Bio-Rad CFX96 instrument. Performance was evaluated for triplicate standard curves of 100,000 to 10 copies ATCC ® synthetic viral RNA diluted in 5 ng/μl human (Jurkat) total RNA (Chikungunya Virus: VR-3246SD; Dengue ...
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WebApr 10, 2024 · After purification, mRNA was resuspended in RNase-free water for use. 2.2. Synthesis of LNP/cLNP. The LNPs were prepared by microfluidic mixing device with baffle mixers. An ethanolic phase containing lipids was mixed with an acidic aqueous phase (50 mM citrate, pH 4.0) containing modified mRNA leading to the formation of LNPs. ... Cy5 … WebIndodicarbocyanine, Cy™5 Technical Information. Cy™5 conjugates are excited maximally at 650 nm and fluoresce maximally at 670 nm. They can be excited to about 98% of … my wa state representative
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WebFigure Legend Snippet: ( A ) A modified Pyrococcus furiosus RNase P RNA ( Pfu RPR ext mP1) labeled with both Cy3 and Cy5 fluorophores. To generate Pfu RPR ext mP1, both … Web$85.10 - $353.00 Description The RNase P gene is a single-copy gene that encodes the RNA moiety for the RNase P enzyme. Applied Biosystems™ TaqMan™ RNase P Control Reagents were designed with limiting primer concentrations to be used as the endogenous reference in multiplex reactions. WebApr 7, 2024 · Besides those specific for SARS-CoV-2 and its variants, the primers and probes targeting the human RNAse P were also used as the internal controls (IC). ... HEX, TexasRED, and CY5) were selected to collect the emitted fluorescent signals. The results were arranged into different patterns, and the variants of SARS-CoV-2 were identified … the simpsons japanese dub